| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 531 | Megasphaera elsdenii | ATGCCGTGACTGCCATCA | TGGAGCAGCTTTTCCGCA | 59.33 | 59.89 | 196 | 40 |
100.00%
|
100.00%
|
| Primer 532 | Megasphaera elsdenii | TGCGGTACAGAATTGCGC | AGGCTGCAACGATGATGACA | 58.83 | 60.04 | 151 | 40 |
100.00%
|
100.00%
|
| Primer 533 | Megasphaera elsdenii | TGCGACGACCCTGCAAAA | TGTGCGCTGCATCGAAGA | 60.20 | 60.05 | 242 | 40 |
100.00%
|
100.00%
|
| Primer 534 | Megasphaera elsdenii | AATGGCAAAACGGCTGGC | CCAGCTTCAACGAGAGCGA | 59.66 | 60.08 | 221 | 40 |
100.00%
|
100.00%
|
| Primer 535 | Megasphaera elsdenii | CCAGGAACGCGATGCCTAT | ATTGACGAACCGTGCCGA | 59.93 | 59.66 | 256 | 40 |
100.00%
|
100.00%
|
| Primer 536 | Megasphaera elsdenii | AAACGCATGACCGTCGCT | ATGTCGTGACGATGCCGA | 60.36 | 59.12 | 241 | 40 |
100.00%
|
100.00%
|
| Primer 537 | Megasphaera elsdenii | ACGACAGCATCAAGGCCA | TGCCGACGATCATGACGA | 59.25 | 58.81 | 207 | 40 |
100.00%
|
100.00%
|
| Primer 538 | Megasphaera elsdenii | ACGACAGCATCAAGGCCA | GTGCCGACGATCATGACGA | 59.25 | 60.23 | 208 | 40 |
100.00%
|
100.00%
|
| Primer 539 | Megasphaera elsdenii | CTGCAACCATCATCGTCGC | AGTTTCGAGTTGCCTCCCG | 59.65 | 60.00 | 157 | 40 |
100.00%
|
100.00%
|
| Primer 540 | Megasphaera elsdenii | ATCGTGTCCAGGAAGGCGA | TGCAACTTTCGTGAACGGC | 60.98 | 59.65 | 257 | 40 |
100.00%
|
100.00%
|